In the present study, researchers deployed a single-component, luminescent BAT biosensor for detecting the SARS-CoV-2 S protein in multiple assay formats. Compared to other single component platforms, they do not count on large conformational changes in the binding module or competition with a tethered decoy. Moreover, they only need a camera for detection and are adapted to multiple assay formats. Split luciferase enzyme-based single-component luminescent biosensors are simpler to produce, highly sensitive, and easier to use. Iii) they require a minimum of two unique antibodies to function. Ii) most antibody-based assays require chemical modification to immobilize the antibodies, and I) antibodies need mammalian expression systems for production, These technologies have three limitations, as follows: Image Credit: NIAID BackgroundĪll the discovery and translational biomedical research depend primarily on antibody-based technologies for small-molecule and protein analyte detection. Study: A single-component luminescent biosensor for the SARS-CoV-2 spike protein. In a recent study posted to the bioRxiv* pre-print server, researchers developed a novel Binding Activated Tandem split enzyme (BAT) biosensor for the detection of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein. By Neha Mathur Reviewed by Danielle Ellis, B.Sc.
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